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GDF15 pretreatment augmented paracrine effects of MSCs. A Schematic showing the experiments for assaying the paracrine effect of MSCs with or without GDF15. B Representative images of tube formation in HUVECs treated with DMEM, MSC-CM or GDF15-MSC-CM. Scale bar = 100 μm. C Quantification of tube formation in HUVECs treated with DMEM, MSC-CM or GDF15-MSC-CM. D The concentration of <t>VEGF,</t> EGF and bFGF in MSC-CM and GDF15-MSC-CM was determined by ELISA assay. E Representative images of the apoptotic NCMs treated with DMEM, MSC-CM or GDF15-MSC-CM under SD/H condition. Scale bar = 200 μm. F Quantification of the apoptotic rate of NCMs treated with DMEM, MSC-CM or GDF15-MSC-CM under SD/H condition. Data are expressed as the mean ± SEM. N = 3 biological replicates for each group. * p < 0.05; ** p < 0.01; *** p < 0.001
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GDF15 pretreatment augmented paracrine effects of MSCs. A Schematic showing the experiments for assaying the paracrine effect of MSCs with or without GDF15. B Representative images of tube formation in HUVECs treated with DMEM, MSC-CM or GDF15-MSC-CM. Scale bar = 100 μm. C Quantification of tube formation in HUVECs treated with DMEM, MSC-CM or GDF15-MSC-CM. D The concentration of VEGF, EGF and bFGF in MSC-CM and GDF15-MSC-CM was determined by ELISA assay. E Representative images of the apoptotic NCMs treated with DMEM, MSC-CM or GDF15-MSC-CM under SD/H condition. Scale bar = 200 μm. F Quantification of the apoptotic rate of NCMs treated with DMEM, MSC-CM or GDF15-MSC-CM under SD/H condition. Data are expressed as the mean ± SEM. N = 3 biological replicates for each group. * p < 0.05; ** p < 0.01; *** p < 0.001

Journal: Stem Cell Research & Therapy

Article Title: Pretreatment with growth differentiation factor 15 augments cardioprotection by mesenchymal stem cells in myocardial infarction by improving their survival

doi: 10.1186/s13287-024-04030-6

Figure Lengend Snippet: GDF15 pretreatment augmented paracrine effects of MSCs. A Schematic showing the experiments for assaying the paracrine effect of MSCs with or without GDF15. B Representative images of tube formation in HUVECs treated with DMEM, MSC-CM or GDF15-MSC-CM. Scale bar = 100 μm. C Quantification of tube formation in HUVECs treated with DMEM, MSC-CM or GDF15-MSC-CM. D The concentration of VEGF, EGF and bFGF in MSC-CM and GDF15-MSC-CM was determined by ELISA assay. E Representative images of the apoptotic NCMs treated with DMEM, MSC-CM or GDF15-MSC-CM under SD/H condition. Scale bar = 200 μm. F Quantification of the apoptotic rate of NCMs treated with DMEM, MSC-CM or GDF15-MSC-CM under SD/H condition. Data are expressed as the mean ± SEM. N = 3 biological replicates for each group. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: The concentration of EGF, VEGF and bFGF in the MSC-CM and GDF15-MSC-CM was examined using a human EGF ELSIA kit (CUSABIO, CSB-E08027h), human VEGF ELSIA kit (CUSABIO, CSB-E11718h), and a human bFGF ELSIA kit (CUSABIO, CSB-E08000h), respectively.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay